Gas Chromatography







Gas chromatography (GC) is a particular chromatography method in which chemical components are separated by a combination of three processes: partition chromatography, adsorption chromatography, difference and boiling point (volatilty in this case) of components in the gaseous state. The substance is passed through a coiled column of glass fused silica oxide-glass located inside a oven. The sample in the column is heated until the components vaporize. Because the molecules possess characteristic size, affinity with the stationary phase, and boiling point, chemical compounds are carried through the column at different times.The period between a compounds injection and their detection by the sensor is called retention time (RT). Retention time is a specific behavior for every compound. Therefore, comparing sample RT with standard RT, the compounds are identified and quantified.